Original paper-EFFECT OF 7-HYDROXY-2-(4-HYDROXY-3-METHOXY-PHENYL)-CHROMAN-4-ONE (SWIETENIA MACROPHYLLA KING SEED) ON RETINOL BINDING PROTEIN-4 AND PHOSPHOENOLPYRUVATE CARBOXYKINASE GENE - ABSTRACT
Authors: Prasetyastuti 1, Sunarti 1, Ahmad Hamim Sadewa 1, Mustofa 2
1 Department of Biochemistry, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
2 Department of Pharmacology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
Background and Aims: Diabetes mellitus is a metabolic disorder characterized by hyperglycemia due to a defect of insulin secretion, insulin action, or both. There are increasing evidence that active compounds of medicinal plants may be used to treat diabetes. The aim of this study is to investigate the effect of a 7-hydroxy-2-(4-hydroxy-3-methoxy-phenyl)-chroman-4-one flavonoid compound of the Swietenia macrophylla King seed on homeostatic model assessment of insulin resistance (HOMA-IR), phosphoenolpyruvate carboxykinase (PEPCK) and retinol binding protein-4 (RBP4) gene expression in diabetic rats. Materials and Method: Thirty Wistar rats were used with 5 in each group as follows: 1) normal rats; 2) diabetic rats; 3) diabetic rats with metformin; 4), 5) and 6) diabetic rats with a 7-hydroxy-2-(4-hydroxy-3-methoxy-phenly)-chroman-4-one 10, 30 and 90 mg/200 g body weight (BW) respectively. Blood glucose and insulin levels were analyzed before and after treatment. At the end of the study, the liver tissue was removed for quantitative PCR (qPCR) analyses. Results: Blood glucose levels decreased significantly (p<0.05) in diabetic rats with metformin and three different dosages of 7-hydroxy-2-(4-hydroxy-3-methoxy-phenly)-chroman-4-one. Insulin levels increased significantly (p<0.05) in diabetic rats with metformin and diabetic rats with 10 mg/200 g BW of 7-hydroxy-2-(4-hydroxy-3-methoxy-phenly)-chroman-4-one. HOMA-IR value decreased significantly (p<0.05) in diabetic rats with metformin and three different dosages of 7-hydroxy-2-(4-hydroxy-3-methoxy-phenly)-chroman-4-one. There was a significant decrease of PEPCK gene expression in the group with 90 mg/200 g BW of 7- hydroxy-2-(4-hydroxy-3-methoxy-phenly)-chroman-4-one. RBP4 gene expression showed a decline, but the difference between groups was not statistically different. Conclusion: These results demonstrated that 7-hydroxy-2-(4-hydroxy-3-methoxy-phenly)-chroman-4-one increased insulin and decreased blood glucose level, HOMA-IR value and PEPCK gene expression.
Key words: Diabetes mellitus, Hyperglycemia, Insulin, PEPCK expression, RBP4- expression, Swietenia macrophyla King seed